Webb Lab

The Webb Lab at IU Indianapolis studies how post-translational modifications (PTMs) reshape protein conformational ensembles and modulate interaction networks critical for cellular function and pathology. Proteins often exist as dynamic populations of interconverting structures rather than single static states. Our research quantifies how chemical modifications such as phosphorylation redistribute these probability landscapes across environments — from dilute solution to complex lysate and chromatin — and how those shifts affect functional interactions. To do this, we integrate proteoform-resolved mass spectrometry, crosslinking-based structural mapping, statistical clustering, and ensemble modeling to move beyond structural snapshots toward mechanistic understanding.